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学术报告——Isotopic Probes for Mechanistic Studies of Reductive Dehydration Enzymatic Reactions

报告题目：Isotopic Probes for Mechanistic Studies of  Reductive Dehydration Enzymatic Reactions

报告人：Youli Xiao , Ph.D.

单 位：Key Laboratory for Synthetic Biology, Institute of Plant Physiology and Ecology，shanghai Institutes for Biological Sciences，CAS

时 间：2012.10.24.(本周三) 下午 2:30

地 点：强磁场中心 201 会议室

报告摘要：

Isoprenoids, the most structurally and functionally diverse group of metabolites with more than 50,000 members in nature, are constructed from two precursors, isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP).  In prokaryotes and plants, these two isoprenoid precursors are supplied by the deoxyxylulose phosphate (DXP) pathway.  The last two steps of the DXP pathway are iron-sulfur cluster catalyzed reductive dehydration reactions.  Recently, using [2-13C, 18O]-MecPP (MEcPP-1), we detected the positional isotopic exchange for the bridging oxygen in MEcPP in presence of IspG without reductants (Scheme 1). In addition, the 13C labelled OH-IPP was converted to the sole product IPP with the retaining isotopic position in the IspH catalyzed reaction (Scheme 2).

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